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Translational_Unit

Part:BBa_K906101:Design

Designed by: Lucas B. Harrington   Group: iGEM12_WashU   (2012-08-13)


psbA2 with Zeaxanthin 7,8-cleavage dioxygenase (ZCD)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PsbA2 is from the genomic sequence preceding the photosystem II D1 protein coding region in Synechocystis spp. 6803. The modified version of ZCD has been codon optimized for the species Synechocystis 6803.


Source

The point mutation (A->T) at the 200th base enables the use of NdeI as mentioned on the main page.

References